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KMID : 1123920120260040469
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Korean Journal of Oriental Physiology and Pathology
2012 Volume.26 No. 4 p.469 ~ p.474
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Fagopyrum esculentum Extract Suppresses the Release of Inflammatory Mediator and Proximal Signal Events in FcεRI-mediated RBL-2H3 Cell Activation
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Kang Kyung-Hwa
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Abstract
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Fagopyrum esculentum(FE) is an important food crop and medicinal plant that is used to improve diabetes, obesity, hypertension, hypercholesterolemia and constipation in Korea, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We investigated the effects on the release of inflammatory mediator and proximal signal events in FcεRI-mediated RBL-2H3 cell activation. FE reduced antigen (DNP?HSA)-induced release of histamine, prostaglandin D2 (PGD2) and cysteinyl Leukotriene (cysLT) in IgE-sensitized RBL-2H3 cells. In addition, it inhibited antigen-induced HDC2 and COX-2 and 5-LO mRNA expression in IgE-sensitized RBL-2H3 cells. FE also suppressed antigen-induced FcεRIβ and FcεRIγ subunit mRNA expression in these cells. To identify the mechanisms underpinning the inhibition of release of inflammatory mediators such as histamine and PGD2 and cysLT by FE, we examined the proximal signal events of intracellular FceRI signaling molecules. FE suppressed antigen-induced phosphorylation of Lyn, Syk, LAT, PLCγ1, PI3K, Akt and cPLA2. Collectively, the anti-allergic effects of FE in vitro suggest its possible therapeutic application to inflammatory allergic diseases, in which its inhibition of inflammatory mediator and FceRI-dependent signaling events in mast cells may be hugely beneficial.
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KEYWORD
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Fagopyrum esculentum (FE), Histamine, PGD2, CysLT, FcεRI-mediated signaling, RBL-2H3
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